Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5401 | 3' | -51.1 | NC_001798.1 | + | 64926 | 0.66 | 0.992511 |
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Target: 5'- aCUGGUCUUCaugGCCGcGAGCaGcAUCAc -3' miRNA: 3'- -GACCAGAAGca-UGGCuUUCGaC-UGGU- -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 78390 | 0.66 | 0.991397 |
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Target: 5'- gCUGGUCg-CGUGCgCGGcggcguugGAGCgcgugGACCAg -3' miRNA: 3'- -GACCAGaaGCAUG-GCU--------UUCGa----CUGGU- -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 116065 | 0.67 | 0.98315 |
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Target: 5'- gCUGGUC-UCGUACaucgugaccuaccuCGggGGC-GACCu -3' miRNA: 3'- -GACCAGaAGCAUG--------------GCuuUCGaCUGGu -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 36053 | 0.7 | 0.941233 |
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Target: 5'- gUGGUCggCGUgcuGCCGGAGGCUG-CgGg -3' miRNA: 3'- gACCAGaaGCA---UGGCUUUCGACuGgU- -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 31300 | 0.7 | 0.920041 |
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Target: 5'- -cGGcCgUCGUGCCGAGAG-UGGCCu -3' miRNA: 3'- gaCCaGaAGCAUGGCUUUCgACUGGu -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 86701 | 0.73 | 0.832881 |
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Target: 5'- -aGGUaUUUGUGCCGAAGcccaccgcGCUGGCCAc -3' miRNA: 3'- gaCCAgAAGCAUGGCUUU--------CGACUGGU- -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 24664 | 0.73 | 0.815308 |
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Target: 5'- cCUGG-CggcCGUGCCG-GGGCUGGCCGg -3' miRNA: 3'- -GACCaGaa-GCAUGGCuUUCGACUGGU- -5' |
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| 5401 | 3' | -51.1 | NC_001798.1 | + | 148595 | 0.73 | 0.797025 |
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Target: 5'- cCUGG-CUUCGcGCCGGuggugcGGCUGACCc -3' miRNA: 3'- -GACCaGAAGCaUGGCUu-----UCGACUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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