Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5414 | 5' | -44.8 | NC_001798.1 | + | 70475 | 0.66 | 0.999999 |
Target: 5'- cCGAG-GCGGccuuccgGGAGAUGgacgUCAUCAAGg -3' miRNA: 3'- aGCUCaCGCU-------UUUUUGCa---AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 57540 | 0.66 | 0.999999 |
Target: 5'- uUCGGGggGCGGAu--GCGUUcCGCgGGGa -3' miRNA: 3'- -AGCUCa-CGCUUuuuUGCAA-GUGgUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 63867 | 0.66 | 0.999998 |
Target: 5'- -aGAG-GCGGAGAGGCcgcccagguuuuccgUCACCAGGu -3' miRNA: 3'- agCUCaCGCUUUUUUGca-------------AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 133598 | 0.66 | 0.999996 |
Target: 5'- cCGGG-GCGggGc-GCGUuaaaaaccUCACCAAGc -3' miRNA: 3'- aGCUCaCGCuuUuuUGCA--------AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 72191 | 0.66 | 0.999995 |
Target: 5'- aUCGAGcagcugcUGCGcGAGcuguACGUgaUCGCCAGGg -3' miRNA: 3'- -AGCUC-------ACGCuUUUu---UGCA--AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 33814 | 0.67 | 0.999994 |
Target: 5'- aUCGGG-GCGAGGGGgcucgcGCGUUCGCa--- -3' miRNA: 3'- -AGCUCaCGCUUUUU------UGCAAGUGguuc -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 50347 | 0.67 | 0.999994 |
Target: 5'- uUUGAGUuuGAAcAGAUGUUUACCGAu -3' miRNA: 3'- -AGCUCAcgCUUuUUUGCAAGUGGUUc -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 111840 | 0.67 | 0.999987 |
Target: 5'- gCGGG-GCGGGAAugcCGUcaaacaggccgcUCACCAGGg -3' miRNA: 3'- aGCUCaCGCUUUUuu-GCA------------AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 109715 | 0.67 | 0.999987 |
Target: 5'- cCGAGcaGCGGGAAcGCGccCGCCGGGg -3' miRNA: 3'- aGCUCa-CGCUUUUuUGCaaGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 95378 | 0.67 | 0.999982 |
Target: 5'- -gGAGgGCGggGcgGCGgUCGCCAGc -3' miRNA: 3'- agCUCaCGCuuUuuUGCaAGUGGUUc -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 111950 | 0.69 | 0.999819 |
Target: 5'- gCGaAGcGCGAGGAAAUGUugacgaUCGCCGGGu -3' miRNA: 3'- aGC-UCaCGCUUUUUUGCA------AGUGGUUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 10754 | 0.69 | 0.999767 |
Target: 5'- cCGGGUGCGuucc-GCGggCGCCGGa -3' miRNA: 3'- aGCUCACGCuuuuuUGCaaGUGGUUc -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 40571 | 0.7 | 0.999621 |
Target: 5'- gCGAcGUGCGAGGGuGCGUagGCCcGAGg -3' miRNA: 3'- aGCU-CACGCUUUUuUGCAagUGG-UUC- -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 59469 | 0.7 | 0.9994 |
Target: 5'- cCGGGcGCGguGAGGCGUUCgGCCAc- -3' miRNA: 3'- aGCUCaCGCuuUUUUGCAAG-UGGUuc -5' |
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5414 | 5' | -44.8 | NC_001798.1 | + | 132028 | 0.75 | 0.984984 |
Target: 5'- cCGGG-GCGaAAAGAACGUUCACguGGu -3' miRNA: 3'- aGCUCaCGC-UUUUUUGCAAGUGguUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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