Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5428 | 3' | -58.5 | NC_001798.1 | + | 72339 | 0.66 | 0.774343 |
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Target: 5'- uGCUGCaGGAgCUCGCcGAGCgCGc- -3' miRNA: 3'- uCGACGaCCUaGAGCGaCUCGgGCac -5' |
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| 5428 | 3' | -58.5 | NC_001798.1 | + | 15534 | 0.66 | 0.76499 |
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Target: 5'- cGCUGCUGGugUUCGUgu-GCCCGg- -3' miRNA: 3'- uCGACGACCuaGAGCGacuCGGGCac -5' |
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| 5428 | 3' | -58.5 | NC_001798.1 | + | 141469 | 0.67 | 0.75552 |
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Target: 5'- gAGCUGCUGaccggCUCGCcgcgcggggggcUGAuGUCCGUGg -3' miRNA: 3'- -UCGACGACcua--GAGCG------------ACU-CGGGCAC- -5' |
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| 5428 | 3' | -58.5 | NC_001798.1 | + | 128438 | 0.67 | 0.706739 |
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Target: 5'- cGCUGCggUGGggCgCGCaGGGUCCGUGc -3' miRNA: 3'- uCGACG--ACCuaGaGCGaCUCGGGCAC- -5' |
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| 5428 | 3' | -58.5 | NC_001798.1 | + | 79922 | 0.68 | 0.676648 |
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Target: 5'- cGCgaguUUGGGUgUCGCUGGGCCCa-- -3' miRNA: 3'- uCGac--GACCUAgAGCGACUCGGGcac -5' |
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| 5428 | 3' | -58.5 | NC_001798.1 | + | 132527 | 0.7 | 0.554206 |
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Target: 5'- gAGCUGCUgaucuacauggacGGGUCcggggCGCgcucgGAGCCCGUc -3' miRNA: 3'- -UCGACGA-------------CCUAGa----GCGa----CUCGGGCAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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