Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5433 | 3' | -56.9 | NC_001798.1 | + | 73615 | 0.7 | 0.677 |
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Target: 5'- cGGGUGUguggUUagGGGGACgaacaGGCGGUUGa -3' miRNA: 3'- aCCCACGa---AG--CCCCUGaa---CUGCCAGC- -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 10280 | 0.71 | 0.626633 |
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Target: 5'- aGGGUGCgUCGGGGGggcGACGGg-- -3' miRNA: 3'- aCCCACGaAGCCCCUgaaCUGCCagc -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 45920 | 0.71 | 0.616541 |
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Target: 5'- cGGGU-C-UCGGGGGg-UGGCGGUCGc -3' miRNA: 3'- aCCCAcGaAGCCCCUgaACUGCCAGC- -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 126598 | 0.72 | 0.576358 |
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Target: 5'- cGGGgcccgcaGCUUCGGGGGCUcggGGgGGUaCGg -3' miRNA: 3'- aCCCa------CGAAGCCCCUGAa--CUgCCA-GC- -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 12033 | 0.72 | 0.556471 |
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Target: 5'- cGGG-GCggCGGGGGCaUG-CGGUCa -3' miRNA: 3'- aCCCaCGaaGCCCCUGaACuGCCAGc -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 113812 | 0.73 | 0.506781 |
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Target: 5'- gUGGGUGC-UCGGGGACagagGAcgccggcCGGUCc -3' miRNA: 3'- -ACCCACGaAGCCCCUGaa--CU-------GCCAGc -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 83269 | 0.74 | 0.470095 |
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Target: 5'- gGGGUGCUg-GGGGuCUUGG-GGUCGc -3' miRNA: 3'- aCCCACGAagCCCCuGAACUgCCAGC- -5' |
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| 5433 | 3' | -56.9 | NC_001798.1 | + | 12890 | 0.75 | 0.416458 |
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Target: 5'- aGGGUGCUgguguaCGGGGGCUUGG-GGcCGu -3' miRNA: 3'- aCCCACGAa-----GCCCCUGAACUgCCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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