Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5449 | 5' | -48.6 | NC_001798.1 | + | 60855 | 0.66 | 0.999575 |
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Target: 5'- -gCGgcGAcGGGAUC-GCGUCCUCc- -3' miRNA: 3'- gaGCauUUaCCUUAGaCGCAGGAGcc -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 74763 | 0.66 | 0.99947 |
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Target: 5'- cCUCGgccccGGAcgCcGCGgCCUCGGg -3' miRNA: 3'- -GAGCauuuaCCUuaGaCGCaGGAGCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 88705 | 0.66 | 0.999343 |
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Target: 5'- -aCGaAAAUGGAGUCcGUGUCCcCGu -3' miRNA: 3'- gaGCaUUUACCUUAGaCGCAGGaGCc -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 130071 | 0.67 | 0.998538 |
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Target: 5'- gUCGUAAAUGGGcUCgGCGUcgggcacaggCC-CGGg -3' miRNA: 3'- gAGCAUUUACCUuAGaCGCA----------GGaGCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 28030 | 0.67 | 0.99824 |
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Target: 5'- aCUCccAAAUGGuccCUGCGUacgaCUCGGg -3' miRNA: 3'- -GAGcaUUUACCuuaGACGCAg---GAGCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 39866 | 0.68 | 0.996918 |
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Target: 5'- gUCGaggcuucgGGggUgCcgGCGUCCUCGGg -3' miRNA: 3'- gAGCauuua---CCuuA-Ga-CGCAGGAGCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 151945 | 0.68 | 0.995168 |
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Target: 5'- -cCGUAAccaaagGUGG--UCUGCGUCCuUUGGa -3' miRNA: 3'- gaGCAUU------UACCuuAGACGCAGG-AGCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 46266 | 0.7 | 0.987168 |
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Target: 5'- gCUCgGUGGAUGaGAAUgucaaagcCUGCGUCCUCc- -3' miRNA: 3'- -GAG-CAUUUAC-CUUA--------GACGCAGGAGcc -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 31260 | 0.71 | 0.971504 |
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Target: 5'- -gCGggGGUGGGAUCUGgGUCUggGGg -3' miRNA: 3'- gaGCauUUACCUUAGACgCAGGagCC- -5' |
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| 5449 | 5' | -48.6 | NC_001798.1 | + | 42645 | 0.73 | 0.949788 |
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Target: 5'- gUCGUGuccAUGGggUCcGUGUCCUCc- -3' miRNA: 3'- gAGCAUu--UACCuuAGaCGCAGGAGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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