Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5461 | 3' | -53.4 | NC_001798.1 | + | 136527 | 0.66 | 0.957428 |
Target: 5'- cCAGAGccgaacGCAGGUCCUUGgaaUUCCAGc-- -3' miRNA: 3'- -GUCUCu-----UGUCCAGGAGC---AAGGUCcau -5' |
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5461 | 3' | -53.4 | NC_001798.1 | + | 105587 | 0.66 | 0.957428 |
Target: 5'- aAGGGuaguACGGGUCCaUGUUCgAGGg- -3' miRNA: 3'- gUCUCu---UGUCCAGGaGCAAGgUCCau -5' |
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5461 | 3' | -53.4 | NC_001798.1 | + | 102233 | 0.67 | 0.949284 |
Target: 5'- gCGGcucGGGGCGGG-CCUCGc-CCGGGUAa -3' miRNA: 3'- -GUC---UCUUGUCCaGGAGCaaGGUCCAU- -5' |
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5461 | 3' | -53.4 | NC_001798.1 | + | 7381 | 0.67 | 0.944848 |
Target: 5'- cCAGAGAACccaGGGUCCcgCGcauacCCAGGg- -3' miRNA: 3'- -GUCUCUUG---UCCAGGa-GCaa---GGUCCau -5' |
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5461 | 3' | -53.4 | NC_001798.1 | + | 67202 | 0.67 | 0.935235 |
Target: 5'- gGGGGGACagccugGGGUCCUUGgggUUCGGGg- -3' miRNA: 3'- gUCUCUUG------UCCAGGAGCa--AGGUCCau -5' |
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5461 | 3' | -53.4 | NC_001798.1 | + | 113826 | 0.74 | 0.624763 |
Target: 5'- aCAGAGGACGccggccGGUCCUCGggccuacgUCUGGGUGu -3' miRNA: 3'- -GUCUCUUGU------CCAGGAGCa-------AGGUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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