Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5467 | 3' | -47.2 | NC_001798.1 | + | 118544 | 0.66 | 0.999904 |
Target: 5'- cGCGGCCCGGcgCcaugaUCAcggAUUGUUu -3' miRNA: 3'- -CGCCGGGCUuaGuag--AGUa--UAACAAc -5' |
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5467 | 3' | -47.2 | NC_001798.1 | + | 106483 | 0.67 | 0.999658 |
Target: 5'- cGCGGCCCGAGgucCAcuUCgCAUAUUa--- -3' miRNA: 3'- -CGCCGGGCUUa--GU--AGaGUAUAAcaac -5' |
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5467 | 3' | -47.2 | NC_001798.1 | + | 4499 | 0.68 | 0.999167 |
Target: 5'- aGCGGCCCGcgucgcGGUCGUCgUCAUcgUcGUc- -3' miRNA: 3'- -CGCCGGGC------UUAGUAG-AGUAuaA-CAac -5' |
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5467 | 3' | -47.2 | NC_001798.1 | + | 117915 | 0.69 | 0.996291 |
Target: 5'- cGCuGCCUGGAgCGUCUCAUcgUGg-- -3' miRNA: 3'- -CGcCGGGCUUaGUAGAGUAuaACaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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