Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5472 | 3' | -56.6 | NC_001798.1 | + | 130436 | 0.69 | 0.720331 |
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Target: 5'- cGACGCggacucccgUCUGGGCCC-CCGg -3' miRNA: 3'- uCUGCGacaacaa--AGACCCGGGcGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 26290 | 0.7 | 0.669146 |
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Target: 5'- -cGCGCUGggGc--CUGGGCgCGCCGc -3' miRNA: 3'- ucUGCGACaaCaaaGACCCGgGCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 2416 | 0.71 | 0.628395 |
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Target: 5'- aGGGCGCcGgcgUGUggCUGGGCCCcggcgGCUGg -3' miRNA: 3'- -UCUGCGaCa--ACAaaGACCCGGG-----CGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 144808 | 0.71 | 0.618195 |
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Target: 5'- cGGCGCgucGUUGUUUcCUGGGUguCgGCCGg -3' miRNA: 3'- uCUGCGa--CAACAAA-GACCCG--GgCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 116701 | 0.72 | 0.577571 |
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Target: 5'- cGGGCGC-GUcGUcgUCgacGGGCCCGCCa -3' miRNA: 3'- -UCUGCGaCAaCAa-AGa--CCCGGGCGGc -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 65575 | 0.73 | 0.517909 |
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Target: 5'- aGGGCGCUGaagaUGUUgucgcagcgCUGGGCCuccuCGCCGg -3' miRNA: 3'- -UCUGCGACa---ACAAa--------GACCCGG----GCGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 71628 | 0.74 | 0.451663 |
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Target: 5'- cAGACGCUGgcGcgcaUCaUGGGCCCgGCCGu -3' miRNA: 3'- -UCUGCGACaaCaa--AG-ACCCGGG-CGGC- -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 93744 | 0.76 | 0.365756 |
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Target: 5'- cGACGCUGaUGcggCUgcGGGCCCGCCa -3' miRNA: 3'- uCUGCGACaACaaaGA--CCCGGGCGGc -5' |
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| 5472 | 3' | -56.6 | NC_001798.1 | + | 75184 | 0.76 | 0.357807 |
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Target: 5'- gAGACGCUGg-------GGGCCCGCCGc -3' miRNA: 3'- -UCUGCGACaacaaagaCCCGGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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