Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5477 | 5' | -43.3 | NC_001798.1 | + | 24776 | 0.66 | 1 |
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Target: 5'- ---cUGCGCGAgcuGCGGU-UCGUGc -3' miRNA: 3'- ccuuAUGUGCUauuUGCCAuAGCACa -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 20927 | 0.66 | 1 |
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Target: 5'- gGGGGaGCAUGAUucGCaGGcGUCGUGUu -3' miRNA: 3'- -CCUUaUGUGCUAuuUG-CCaUAGCACA- -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 15470 | 0.69 | 0.999985 |
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Target: 5'- uGggUAgACG-UGGGCGGggggGUCGUGg -3' miRNA: 3'- cCuuAUgUGCuAUUUGCCa---UAGCACa -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 19601 | 0.69 | 0.999985 |
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Target: 5'- ---uUACACGuacGAGCGGUGUCGcGUu -3' miRNA: 3'- ccuuAUGUGCua-UUUGCCAUAGCaCA- -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 116024 | 0.7 | 0.999961 |
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Target: 5'- uGGAAcaacACGCGAUGcGCGGcguUCGUGa -3' miRNA: 3'- -CCUUa---UGUGCUAUuUGCCau-AGCACa -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 119968 | 0.7 | 0.999948 |
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Target: 5'- cGGAGUACAUGcgGAcguCGGUGUUGg-- -3' miRNA: 3'- -CCUUAUGUGCuaUUu--GCCAUAGCaca -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 110607 | 0.71 | 0.99993 |
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Target: 5'- uGGAG-ACGCGaAUAAACGGg--UGUGUg -3' miRNA: 3'- -CCUUaUGUGC-UAUUUGCCauaGCACA- -5' |
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| 5477 | 5' | -43.3 | NC_001798.1 | + | 41519 | 0.71 | 0.99984 |
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Target: 5'- ----cACACGA-GGGCGGUAUCGuUGUg -3' miRNA: 3'- ccuuaUGUGCUaUUUGCCAUAGC-ACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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