Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5481 | 5' | -59 | NC_001798.1 | + | 23420 | 0.66 | 0.768341 |
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Target: 5'- cUCCUCCUccGCcuccUCCuCCUCGUCUgcaUCCGc -3' miRNA: 3'- -AGGAGGA--CGu---AGG-GGAGCGGA---AGGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 46283 | 0.67 | 0.710369 |
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Target: 5'- gUCaaagCCUGCGUCCuCCUCGCgCUUUgGc -3' miRNA: 3'- -AGga--GGACGUAGG-GGAGCG-GAAGgUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 152016 | 0.68 | 0.660172 |
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Target: 5'- cUCCUcCCUGCc-UCCCUCGCCcccCCAg -3' miRNA: 3'- -AGGA-GGACGuaGGGGAGCGGaa-GGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 42441 | 0.68 | 0.660172 |
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Target: 5'- gCCUgCUGgAUCaCCCUCGCCaccCCAc -3' miRNA: 3'- aGGAgGACgUAG-GGGAGCGGaa-GGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 94205 | 0.68 | 0.650032 |
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Target: 5'- cCCUgCUGCAgCUCCUCGCgCggcggUCCAa -3' miRNA: 3'- aGGAgGACGUaGGGGAGCG-Ga----AGGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 31942 | 0.7 | 0.549111 |
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Target: 5'- uUCCUCCgccgccccGCGcucgCCCCUCGCCccCCAg -3' miRNA: 3'- -AGGAGGa-------CGUa---GGGGAGCGGaaGGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 118515 | 0.7 | 0.53922 |
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Target: 5'- cCCUCCcGCA-CCCC-CGCgUUCCGg -3' miRNA: 3'- aGGAGGaCGUaGGGGaGCGgAAGGUa -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 22431 | 0.7 | 0.509937 |
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Target: 5'- cUCCUCCUccGCGUCCuCCUC-CUUUCCc- -3' miRNA: 3'- -AGGAGGA--CGUAGG-GGAGcGGAAGGua -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 562 | 0.71 | 0.471981 |
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Target: 5'- cCCUCCcGCG-CCCCgUGCCUUCCc- -3' miRNA: 3'- aGGAGGaCGUaGGGGaGCGGAAGGua -5' |
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| 5481 | 5' | -59 | NC_001798.1 | + | 146189 | 1.05 | 0.002409 |
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Target: 5'- cUCCUCCUGCAUCCCCUCGCCUUCCAUu -3' miRNA: 3'- -AGGAGGACGUAGGGGAGCGGAAGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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