Results 81 - 86 of 86 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5483 | 3' | -57.3 | NC_001798.1 | + | 52273 | 0.67 | 0.806881 |
Target: 5'- uCCGCCCACCccgcguuugcGGACGuGGAGCaGGAGg -3' miRNA: 3'- -GGUGGGUGGu---------UCUGUcCCUCGgUCUU- -5' |
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5483 | 3' | -57.3 | NC_001798.1 | + | 7063 | 0.67 | 0.806881 |
Target: 5'- cCCACCC-CCAaaccccgguugGGGCGGGcAGCCgAGAc -3' miRNA: 3'- -GGUGGGuGGU-----------UCUGUCCcUCGG-UCUu -5' |
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5483 | 3' | -57.3 | NC_001798.1 | + | 2257 | 0.67 | 0.805997 |
Target: 5'- gCGCgCCGCCGggGGGCGGGGcggcgcagcgcgcGGCCAGc- -3' miRNA: 3'- gGUG-GGUGGU--UCUGUCCC-------------UCGGUCuu -5' |
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5483 | 3' | -57.3 | NC_001798.1 | + | 135207 | 0.67 | 0.788909 |
Target: 5'- gCCGCCCuCgCGGGccuCGGGGAGgCCGGGc -3' miRNA: 3'- -GGUGGGuG-GUUCu--GUCCCUC-GGUCUu -5' |
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5483 | 3' | -57.3 | NC_001798.1 | + | 74640 | 0.67 | 0.788909 |
Target: 5'- aCCgGCCCcCCGAGGCcccccGGGGGGCCucGGGc -3' miRNA: 3'- -GG-UGGGuGGUUCUG-----UCCCUCGG--UCUu -5' |
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5483 | 3' | -57.3 | NC_001798.1 | + | 125783 | 0.68 | 0.760915 |
Target: 5'- gCACCUccggGCCGAGguguACAGGGA-CCAGAc -3' miRNA: 3'- gGUGGG----UGGUUC----UGUCCCUcGGUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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