Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5486 | 3' | -53.5 | NC_001798.1 | + | 27795 | 0.66 | 0.968673 |
Target: 5'- gGGuGAGUUCGCUaGGCAaGCAcGgACu -3' miRNA: 3'- -CCuCUCAAGUGAgCCGUgCGUaCgUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 76257 | 0.66 | 0.968673 |
Target: 5'- cGGGAcgUCGCUCaGCGCGCc-GCGCu -3' miRNA: 3'- cCUCUcaAGUGAGcCGUGCGuaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 149418 | 0.66 | 0.965488 |
Target: 5'- cGGGGGGccUCACgcaguuGCGCGCGUGCuCg -3' miRNA: 3'- -CCUCUCa-AGUGagc---CGUGCGUACGuG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 92299 | 0.66 | 0.965488 |
Target: 5'- uGGAGcugcugUCGCuacugUCGGCGCGCAgcgGCGa -3' miRNA: 3'- -CCUCuca---AGUG-----AGCCGUGCGUa--CGUg -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 34091 | 0.66 | 0.965488 |
Target: 5'- gGGGGGGUg-AC-CGGCGCGUggGgGCg -3' miRNA: 3'- -CCUCUCAagUGaGCCGUGCGuaCgUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 60788 | 0.66 | 0.962082 |
Target: 5'- aGGAucccGUUCAgCUCGGCGCccuGCA-GCACu -3' miRNA: 3'- -CCUcu--CAAGU-GAGCCGUG---CGUaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 143081 | 0.66 | 0.954584 |
Target: 5'- uGGAGGcucuugUUGCUgcgUGGUACGCAUGUGCg -3' miRNA: 3'- -CCUCUca----AGUGA---GCCGUGCGUACGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 68432 | 0.66 | 0.954584 |
Target: 5'- uGGGGGUUCgugACUUGGCuCGCcgaGCACc -3' miRNA: 3'- cCUCUCAAG---UGAGCCGuGCGua-CGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 43589 | 0.67 | 0.950483 |
Target: 5'- cGGAu-GUUCAgCUgGGCGCGCAcGUGCc -3' miRNA: 3'- -CCUcuCAAGU-GAgCCGUGCGUaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 109197 | 0.67 | 0.946142 |
Target: 5'- gGGGGAGgacccggGCUcCGGCACGgAcGCGCg -3' miRNA: 3'- -CCUCUCaag----UGA-GCCGUGCgUaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 27161 | 0.67 | 0.940612 |
Target: 5'- gGGAGGGgcugcugcgaGCUCGGgGcCGCggGCGCg -3' miRNA: 3'- -CCUCUCaag-------UGAGCCgU-GCGuaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 32833 | 0.67 | 0.936728 |
Target: 5'- gGGGGAGcuaaCACUCGGCuuGC-UGCcCg -3' miRNA: 3'- -CCUCUCaa--GUGAGCCGugCGuACGuG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 85976 | 0.68 | 0.926327 |
Target: 5'- cGGGGAGUUCgaggccgccGC-CGGgGCGCugGCGCa -3' miRNA: 3'- -CCUCUCAAG---------UGaGCCgUGCGuaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 35133 | 0.68 | 0.925232 |
Target: 5'- gGGAGAGgagaag-GGCGCGCcgGUGCGCg -3' miRNA: 3'- -CCUCUCaagugagCCGUGCG--UACGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 1655 | 0.68 | 0.895996 |
Target: 5'- -cGGGGU--ACa-GGCGCGCGUGCGCg -3' miRNA: 3'- ccUCUCAagUGagCCGUGCGUACGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 36484 | 0.69 | 0.885012 |
Target: 5'- gGGAGGGggCGC-CGGCGCgacgcgggcggccggGCGgggGCGCg -3' miRNA: 3'- -CCUCUCaaGUGaGCCGUG---------------CGUa--CGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 154040 | 0.69 | 0.882172 |
Target: 5'- uGGGAGcgCGC-CGGgGCGCG-GCACg -3' miRNA: 3'- cCUCUCaaGUGaGCCgUGCGUaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 149247 | 0.69 | 0.874911 |
Target: 5'- cGGGGGcgUCGC-CGGCcgGCGCggGCGCg -3' miRNA: 3'- cCUCUCa-AGUGaGCCG--UGCGuaCGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 126727 | 0.69 | 0.874911 |
Target: 5'- gGGGGAGUgCGCUCuuCGCGCGgccGCACc -3' miRNA: 3'- -CCUCUCAaGUGAGccGUGCGUa--CGUG- -5' |
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5486 | 3' | -53.5 | NC_001798.1 | + | 26260 | 0.69 | 0.85972 |
Target: 5'- aGGAcGAGgcgCACUCGcacCGCGCcUGCGCg -3' miRNA: 3'- -CCU-CUCaa-GUGAGCc--GUGCGuACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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