miRNA: 3'- uaauaaUCCCGUu---UCACgCuCGUGAC- -5'
miRNA: 3'- uaauaaUCCCGUUUCaCGC-UCGUGac -5'
miRNA: 3'- uaauaaUCCCGUUUCa-CGC-UCGUGac -5'
miRNA: 3'- uaauaaUCCCGUU-UCACGCUCgUGac -5'
miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5489 | 5' | -48.6 | NC_001798.1 | + | 32717 | 0.67 | 0.995807 |
|
Target: 5'- -----cAGGGCAcccuGGUGgGcGCGCUGg -3' miRNA: 3'- uaauaaUCCCGUu---UCACgCuCGUGAC- -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 97537 | 0.67 | 0.995071 |
|
Target: 5'- -----gGGGGCGGAG-GCGuGGCGCg- -3' miRNA: 3'- uaauaaUCCCGUUUCaCGC-UCGUGac -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 2268 | 0.67 | 0.995071 |
|
Target: 5'- -----gGGGGCGGGGcgGCGcAGCGCg- -3' miRNA: 3'- uaauaaUCCCGUUUCa-CGC-UCGUGac -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 31152 | 0.67 | 0.993285 |
|
Target: 5'- -----gGGGGCGGcGGUGCGGGgGCg- -3' miRNA: 3'- uaauaaUCCCGUU-UCACGCUCgUGac -5' |
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| 5489 | 5' | -48.6 | NC_001798.1 | + | 5842 | 1.08 | 0.013515 |
|
Target: 5'- uAUUAUUAGGGCAAAGUGCGAGCACUGg -3' miRNA: 3'- -UAAUAAUCCCGUUUCACGCUCGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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