Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5505 | 3' | -56.3 | NC_001798.1 | + | 59672 | 0.66 | 0.898097 |
Target: 5'- cGACUCGGCUCgcgUGGGGGcggcgggcacgCCCGu -3' miRNA: 3'- cCUGAGCCGGGagaAUCUCCua---------GGGC- -5' |
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5505 | 3' | -56.3 | NC_001798.1 | + | 69727 | 0.66 | 0.899371 |
Target: 5'- cGGGCgaaggCGGCuuccgCCUC-UGGuGGGUUCCGg -3' miRNA: 3'- -CCUGa----GCCG-----GGAGaAUCuCCUAGGGC- -5' |
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5505 | 3' | -56.3 | NC_001798.1 | + | 107412 | 0.66 | 0.917382 |
Target: 5'- aGGACUgGGgCCggCUgacGGGGGUCgCCGc -3' miRNA: 3'- -CCUGAgCCgGGa-GAau-CUCCUAG-GGC- -5' |
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5505 | 3' | -56.3 | NC_001798.1 | + | 152052 | 0.66 | 0.922924 |
Target: 5'- gGGGCggcgcaCGGCCCac---GGGGGUCCCc -3' miRNA: 3'- -CCUGa-----GCCGGGagaauCUCCUAGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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