Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5505 | 5' | -56.3 | NC_001798.1 | + | 28278 | 0.69 | 0.679223 |
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Target: 5'- -cCGCAGcgCCCCCUuccgccccacCUGGCGCu -3' miRNA: 3'- aaGCGUCuaGGGGGGuuu-------GACCGUGu -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 53176 | 0.7 | 0.670966 |
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Target: 5'- -aCGCGGGcgUCCCCGAGC-GGCGCAu -3' miRNA: 3'- aaGCGUCUagGGGGGUUUGaCCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 39357 | 0.7 | 0.639855 |
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Target: 5'- aUCGCAGAaCCgCCCGAAC-GGCGgGg -3' miRNA: 3'- aAGCGUCUaGGgGGGUUUGaCCGUgU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 122390 | 0.71 | 0.608686 |
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Target: 5'- -cCGCGGcugUCCUCCGAGCUGGcCACGg -3' miRNA: 3'- aaGCGUCua-GGGGGGUUUGACC-GUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 75240 | 0.71 | 0.587984 |
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Target: 5'- gUUCG-AGGUCCaCCCCAAugUGGC-CGc -3' miRNA: 3'- -AAGCgUCUAGG-GGGGUUugACCGuGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 2888 | 0.71 | 0.567413 |
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Target: 5'- -gCGCAGGUCCCCgC--GCaGGCGCAu -3' miRNA: 3'- aaGCGUCUAGGGGgGuuUGaCCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 152624 | 0.72 | 0.516916 |
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Target: 5'- -aCGCAGAugggagUCCCCCCGGcgccccGCcGGCGCGg -3' miRNA: 3'- aaGCGUCU------AGGGGGGUU------UGaCCGUGU- -5' |
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| 5505 | 5' | -56.3 | NC_001798.1 | + | 28165 | 0.73 | 0.495261 |
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Target: 5'- -aCGCAGAgugggcgaacagCCCCCCGAGCgagGGCGgGg -3' miRNA: 3'- aaGCGUCUa-----------GGGGGGUUUGa--CCGUgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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