Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5519 | 3' | -56.4 | NC_001798.1 | + | 146082 | 0.75 | 0.437112 |
Target: 5'- aGGGGUC-Cgg-GGCGagGCGGGCGGGc -3' miRNA: 3'- -UCCCAGaGuaaUCGUagCGCCCGCCU- -5' |
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5519 | 3' | -56.4 | NC_001798.1 | + | 5434 | 0.75 | 0.410628 |
Target: 5'- cGGcaUCUCAUUAGCGcccggCGCGGGCGGc -3' miRNA: 3'- uCCc-AGAGUAAUCGUa----GCGCCCGCCu -5' |
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5519 | 3' | -56.4 | NC_001798.1 | + | 145785 | 0.75 | 0.402024 |
Target: 5'- cGGGGUCcacaCAggAGCG-CGCGGGCGGc -3' miRNA: 3'- -UCCCAGa---GUaaUCGUaGCGCCCGCCu -5' |
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5519 | 3' | -56.4 | NC_001798.1 | + | 22227 | 1.09 | 0.002541 |
Target: 5'- gAGGGUCUCAUUAGCAUCGCGGGCGGAa -3' miRNA: 3'- -UCCCAGAGUAAUCGUAGCGCCCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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