Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5522 | 5' | -53.5 | NC_001798.1 | + | 2254 | 0.66 | 0.963915 |
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Target: 5'- ---cGGGCGcgccgccGGGGGGCGG-GGCGGCGc -3' miRNA: 3'- aauaCCCGU-------CCUCUUGCUgCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 52504 | 0.66 | 0.960773 |
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Target: 5'- ---cGGcGCAGGG--ACGACG-CGGCGg -3' miRNA: 3'- aauaCC-CGUCCUcuUGCUGCuGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 85814 | 0.66 | 0.960773 |
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Target: 5'- ---gGGGCgaugacggGGGGGGGCGGgGGCGgGCGg -3' miRNA: 3'- aauaCCCG--------UCCUCUUGCUgCUGC-UGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 73620 | 0.66 | 0.957067 |
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Target: 5'- gUGUGGuuAGGGGGACGAacaGGCGGu- -3' miRNA: 3'- aAUACCcgUCCUCUUGCUg--CUGCUgc -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 58799 | 0.66 | 0.970551 |
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Target: 5'- ---gGGGCgaggaggaaaAGGGGAugGACGuCGuCGg -3' miRNA: 3'- aauaCCCG----------UCCUCUugCUGCuGCuGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 111183 | 0.66 | 0.957067 |
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Target: 5'- --uUGGGCGGGAGGAgGuCGAacaUGAgGa -3' miRNA: 3'- aauACCCGUCCUCUUgCuGCU---GCUgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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