Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5522 | 5' | -53.5 | NC_001798.1 | + | 122233 | 0.66 | 0.96751 |
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Target: 5'- ---cGGGUGGGAagcucuGGcCGGCGGCGACc -3' miRNA: 3'- aauaCCCGUCCU------CUuGCUGCUGCUGc -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 73943 | 0.66 | 0.96751 |
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Target: 5'- --cUGGuGgAGGGGGGCGA-GugGACGc -3' miRNA: 3'- aauACC-CgUCCUCUUGCUgCugCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 21954 | 0.66 | 0.96751 |
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Target: 5'- --uUGGGC-GGAGcGCGG-GAUGACGc -3' miRNA: 3'- aauACCCGuCCUCuUGCUgCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 81079 | 0.66 | 0.96996 |
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Target: 5'- uUUGUGGcuaccGCGGGGGAcguucucGCGGCGAgcgccgcCGACGc -3' miRNA: 3'- -AAUACC-----CGUCCUCU-------UGCUGCU-------GCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 35837 | 0.66 | 0.970551 |
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Target: 5'- ---aGGGCcGGAGAAUGgaaGGCGAgGg -3' miRNA: 3'- aauaCCCGuCCUCUUGCug-CUGCUgC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 58799 | 0.66 | 0.970551 |
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Target: 5'- ---gGGGCgaggaggaaaAGGGGAugGACGuCGuCGg -3' miRNA: 3'- aauaCCCG----------UCCUCUugCUGCuGCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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