Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5522 | 5' | -53.5 | NC_001798.1 | + | 2750 | 0.68 | 0.906772 |
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Target: 5'- --cUGGaagaGCAGGuccGCGGCGGCGGCGg -3' miRNA: 3'- aauACC----CGUCCucuUGCUGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 2686 | 0.69 | 0.864995 |
|
Target: 5'- ---cGGGCGcGGcGAGCGagucggccGCGGCGACGg -3' miRNA: 3'- aauaCCCGU-CCuCUUGC--------UGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 2538 | 0.66 | 0.964252 |
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Target: 5'- ---gGGGCGGGGGGcGCGGCccccgcgggaggGGCGGCc -3' miRNA: 3'- aauaCCCGUCCUCU-UGCUG------------CUGCUGc -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 2507 | 0.7 | 0.841116 |
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Target: 5'- ---gGGGCGcGGcGGccGCGGCGGCGGCGu -3' miRNA: 3'- aauaCCCGU-CCuCU--UGCUGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 2254 | 0.66 | 0.963915 |
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Target: 5'- ---cGGGCGcgccgccGGGGGGCGG-GGCGGCGc -3' miRNA: 3'- aauaCCCGU-------CCUCUUGCUgCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 52 | 0.75 | 0.56544 |
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Target: 5'- --uUGGGgGGGGGcgcgaaggcgGGCGGCGGCGGCGg -3' miRNA: 3'- aauACCCgUCCUC----------UUGCUGCUGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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