Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5522 | 5' | -53.5 | NC_001798.1 | + | 35430 | 0.68 | 0.929831 |
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Target: 5'- -aAUGcGGCGGGAG-GCGugGGCcgcuGGCGc -3' miRNA: 3'- aaUAC-CCGUCCUCuUGCugCUG----CUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 11321 | 0.68 | 0.929831 |
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Target: 5'- ----aGGCAuGGucGugGACGACGACGa -3' miRNA: 3'- aauacCCGU-CCucUugCUGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 74207 | 0.68 | 0.929831 |
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Target: 5'- --cUGGGCAuGGggcucuaccGGGACGGCGAC-ACGg -3' miRNA: 3'- aauACCCGU-CC---------UCUUGCUGCUGcUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 42191 | 0.68 | 0.928238 |
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Target: 5'- -cAUGGGCGGGgcgugaaaguGGAagcgccgcgggucgGCGugcGCGGCGACGa -3' miRNA: 3'- aaUACCCGUCC----------UCU--------------UGC---UGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 128583 | 0.68 | 0.924435 |
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Target: 5'- -cGUGGGCcgcgcGGAcgcGGGCGucaACGACGACGc -3' miRNA: 3'- aaUACCCGu----CCU---CUUGC---UGCUGCUGC- -5' |
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| 5522 | 5' | -53.5 | NC_001798.1 | + | 88060 | 0.69 | 0.879843 |
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Target: 5'- ---gGGGCGGGcucguccccuGGGGCGGCGGCGuCu -3' miRNA: 3'- aauaCCCGUCC----------UCUUGCUGCUGCuGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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