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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5534 | 5' | -54.7 | NC_001806.1 | + | 21008 | 0.68 | 0.866009 |
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Target: 5'- gGUCCg-UGGGUCUCGcCCCCUCc--- -3' miRNA: 3'- -CAGGaaACCUAGGGCuGGGGAGaaga -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 32716 | 0.73 | 0.630287 |
|
Target: 5'- uGUCgUUUGGGUCCCc-CCCCUCUa-- -3' miRNA: 3'- -CAGgAAACCUAGGGcuGGGGAGAaga -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 49673 | 0.69 | 0.808102 |
|
Target: 5'- uUCCcgUUGGAUCgaCUGGCCCCUCa--- -3' miRNA: 3'- cAGGa-AACCUAG--GGCUGGGGAGaaga -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 67495 | 0.74 | 0.53815 |
|
Target: 5'- cUCCg--GGGUCCCGACCCC-CUg-- -3' miRNA: 3'- cAGGaaaCCUAGGGCUGGGGaGAaga -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 111484 | 0.7 | 0.799113 |
|
Target: 5'- -gCCUuuUUGGGUCCgGGCCCgUCgugUCUu -3' miRNA: 3'- caGGA--AACCUAGGgCUGGGgAGa--AGA- -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 149195 | 1.07 | 0.004743 |
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Target: 5'- cGUCCUUUGGAUCCCGACCCCUCUUCUu -3' miRNA: 3'- -CAGGAAACCUAGGGCUGGGGAGAAGA- -5' |
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| 5534 | 5' | -54.7 | NC_001806.1 | + | 149776 | 0.68 | 0.873475 |
|
Target: 5'- -----aUGGcgCCCGGCCCCUCa--- -3' miRNA: 3'- caggaaACCuaGGGCUGGGGAGaaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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