Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5540 | 3' | -64.1 | NC_001806.1 | + | 77375 | 0.66 | 0.51316 |
|
Target: 5'- aCAGGcCAGaCgGGcGCGGGCGcGGCGg- -3' miRNA: 3'- -GUCCaGUCaGgCC-CGCCCGU-CCGCga -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 34775 | 0.66 | 0.51316 |
|
Target: 5'- gCGGGgCGGUUCGGGgGGGgGGGgGg- -3' miRNA: 3'- -GUCCaGUCAGGCCCgCCCgUCCgCga -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 31953 | 0.66 | 0.51316 |
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Target: 5'- uGGGagGGguggCgGGGCGGGacgGGGCGCc -3' miRNA: 3'- gUCCagUCa---GgCCCGCCCg--UCCGCGa -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 83276 | 0.66 | 0.502977 |
|
Target: 5'- gAGGg-GGUUCGGGCGGGCGcGGaggacccCGCa -3' miRNA: 3'- gUCCagUCAGGCCCGCCCGU-CC-------GCGa -5' |
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| 5540 | 3' | -64.1 | NC_001806.1 | + | 56852 | 0.65 | 0.559453 |
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Target: 5'- cCAGGgCGG-CCGcGGCGGGCccaccgauccaccAGaGCGCg -3' miRNA: 3'- -GUCCaGUCaGGC-CCGCCCG-------------UC-CGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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