Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5546 | 5' | -60 | NC_001806.1 | + | 4970 | 0.7 | 0.51957 |
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Target: 5'- cGUCCCGCcggGCgucgucgagucgUGGgggUGGUCGGGGUCGUg -3' miRNA: 3'- -CGGGGCG---CGa-----------ACU---GCCAGUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 151063 | 0.7 | 0.512011 |
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Target: 5'- cGCaCCCGCGC--GGgGGUCgcgGGGGUCGc -3' miRNA: 3'- -CG-GGGCGCGaaCUgCCAG---UCCCAGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 151251 | 0.7 | 0.511069 |
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Target: 5'- aGCCCCGCcccccgggcccacGCcgGGCGGUgGGGGcCGg -3' miRNA: 3'- -CGGGGCG-------------CGaaCUGCCAgUCCCaGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 132695 | 0.72 | 0.430585 |
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Target: 5'- uGgCCCGCGg-UGGgGGcCAGGGUCGUc -3' miRNA: 3'- -CgGGGCGCgaACUgCCaGUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 29803 | 0.74 | 0.334848 |
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Target: 5'- cGCCgCCGCGCcccccgUGACGGgCGGGG-CGUc -3' miRNA: 3'- -CGG-GGCGCGa-----ACUGCCaGUCCCaGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 42010 | 0.75 | 0.267684 |
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Target: 5'- uGCUCCGUGC-UGGCcgcggGGUUGGGGUCGUg -3' miRNA: 3'- -CGGGGCGCGaACUG-----CCAGUCCCAGCA- -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 31357 | 0.76 | 0.261601 |
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Target: 5'- cCCCCGUGUUUGugGGgaggggGGGGUCGg -3' miRNA: 3'- cGGGGCGCGAACugCCag----UCCCAGCa -5' |
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| 5546 | 5' | -60 | NC_001806.1 | + | 141500 | 1.09 | 0.001286 |
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Target: 5'- cGCCCCGCGCUUGACGGUCAGGGUCGUg -3' miRNA: 3'- -CGGGGCGCGAACUGCCAGUCCCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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