Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5547 | 3' | -56.9 | NC_001806.1 | + | 136737 | 0.66 | 0.871488 |
Target: 5'- cGAGGucCCGGUaagUGGUGGCGUUgaGGa -3' miRNA: 3'- -CUCCcaGGCCGg--ACUACCGUAAgaCC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 105583 | 0.66 | 0.856469 |
Target: 5'- cGGGGUUCGGUCccagGGcUGGCAcUCUGu -3' miRNA: 3'- cUCCCAGGCCGGa---CU-ACCGUaAGACc -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 122936 | 0.66 | 0.848655 |
Target: 5'- cAGGGaccaCGGUCUGGUGGCGc-UUGGg -3' miRNA: 3'- cUCCCag--GCCGGACUACCGUaaGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 65641 | 0.67 | 0.839835 |
Target: 5'- aGAGGGgccccggCGGCCUcccccugGGUGGCugcgCUGGg -3' miRNA: 3'- -CUCCCag-----GCCGGA-------CUACCGuaa-GACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 67205 | 0.67 | 0.832451 |
Target: 5'- --cGGUcugCCGGCCUGGcggGGCGcgCUGGu -3' miRNA: 3'- cucCCA---GGCCGGACUa--CCGUaaGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 11836 | 0.67 | 0.80681 |
Target: 5'- aGGGGGUggaGGCCUGGUuaggGGCGggUUGGu -3' miRNA: 3'- -CUCCCAgg-CCGGACUA----CCGUaaGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 119488 | 0.67 | 0.80681 |
Target: 5'- cGGGcGUgCGGCCUG-UGGCGUugcaUCgGGg -3' miRNA: 3'- cUCC-CAgGCCGGACuACCGUA----AGaCC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 133462 | 0.68 | 0.797939 |
Target: 5'- -cGGGUCCugGGCCUGAcaacGGCAUgcgugCcGGa -3' miRNA: 3'- cuCCCAGG--CCGGACUa---CCGUAa----GaCC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 69605 | 0.68 | 0.788919 |
Target: 5'- cGAGGcGgUCGGCCUGucggggGGCGUUCUa- -3' miRNA: 3'- -CUCC-CaGGCCGGACua----CCGUAAGAcc -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 69658 | 0.68 | 0.788919 |
Target: 5'- -uGGGUCCGGCCgugccGAcGGaacaUCUGGc -3' miRNA: 3'- cuCCCAGGCCGGa----CUaCCgua-AGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 117365 | 0.68 | 0.77976 |
Target: 5'- cGGaGGUCCGGCCUGGgccgcgaGGUAga-UGGc -3' miRNA: 3'- cUC-CCAGGCCGGACUa------CCGUaagACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 103708 | 0.68 | 0.751531 |
Target: 5'- uGGGGGUuuGGUCggcgGGUGGUAa-CUGGc -3' miRNA: 3'- -CUCCCAggCCGGa---CUACCGUaaGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 99639 | 0.69 | 0.712478 |
Target: 5'- uGGGGGcgguggCgGGCCUGGcgGGCAggcagCUGGg -3' miRNA: 3'- -CUCCCa-----GgCCGGACUa-CCGUaa---GACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 31377 | 0.7 | 0.652041 |
Target: 5'- gGGGGGUCgGGCgCUgGGUGGUc-UCUGGc -3' miRNA: 3'- -CUCCCAGgCCG-GA-CUACCGuaAGACC- -5' |
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5547 | 3' | -56.9 | NC_001806.1 | + | 141366 | 1.11 | 0.001665 |
Target: 5'- cGAGGGUCCGGCCUGAUGGCAUUCUGGg -3' miRNA: 3'- -CUCCCAGGCCGGACUACCGUAAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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