Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5551 | 3' | -62.7 | NC_001806.1 | + | 41422 | 0.68 | 0.473063 |
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Target: 5'- cCUGCCCagcGCGCCGUGgucGG-GGUGCGc -3' miRNA: 3'- -GACGGGc--UGCGGCGCgu-CCaCCACGU- -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 22773 | 0.68 | 0.473063 |
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Target: 5'- -cGCCgGGCGCCGCGCccAGGccGGccGCGu -3' miRNA: 3'- gaCGGgCUGCGGCGCG--UCCa-CCa-CGU- -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 76283 | 0.68 | 0.455184 |
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Target: 5'- cCUGgCCGcCGCCGCGgAGGagauauccgcGGUGCGc -3' miRNA: 3'- -GACgGGCuGCGGCGCgUCCa---------CCACGU- -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 100387 | 0.69 | 0.446382 |
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Target: 5'- -cGCCgGGCGgCGCGgGGGccggGGUGCc -3' miRNA: 3'- gaCGGgCUGCgGCGCgUCCa---CCACGu -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 139112 | 0.69 | 0.437676 |
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Target: 5'- gCUGaCCGGCucGCCGCGCGGG-GG-GCu -3' miRNA: 3'- -GACgGGCUG--CGGCGCGUCCaCCaCGu -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 106543 | 0.69 | 0.437676 |
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Target: 5'- -gGUgCGAUGCCGCGCuGGUGcGcGCGc -3' miRNA: 3'- gaCGgGCUGCGGCGCGuCCAC-CaCGU- -5' |
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| 5551 | 3' | -62.7 | NC_001806.1 | + | 139765 | 1.08 | 0.000821 |
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Target: 5'- aCUGCCCGACGCCGCGCAGGUGGUGCAu -3' miRNA: 3'- -GACGGGCUGCGGCGCGUCCACCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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