Results 61 - 68 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5553 | 3' | -56.4 | NC_001806.1 | + | 3727 | 0.74 | 0.460389 |
Target: 5'- gGGCGCGUagGCGCgGCGcAGGcUGguCAGCa -3' miRNA: 3'- -CCGUGUA--CGCGaCGC-UCC-ACguGUCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 59182 | 0.74 | 0.450225 |
Target: 5'- gGGCGUAUGcCGCUGCGAGGgugggGUuuugaugGCAGCc -3' miRNA: 3'- -CCGUGUAC-GCGACGCUCCa----CG-------UGUCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 109691 | 0.75 | 0.397925 |
Target: 5'- gGGCugGUuCGCaGCGAGGUGCAguacuCGGCc -3' miRNA: 3'- -CCGugUAcGCGaCGCUCCACGU-----GUCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 2849 | 0.76 | 0.37287 |
Target: 5'- gGGCGCggGCGCgGgGAGGgggGCGCGGg -3' miRNA: 3'- -CCGUGuaCGCGaCgCUCCa--CGUGUCg -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 95290 | 0.76 | 0.348909 |
Target: 5'- cGCGCAUGgGg-GCGGGGgGCGCGGCg -3' miRNA: 3'- cCGUGUACgCgaCGCUCCaCGUGUCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 145030 | 0.76 | 0.340402 |
Target: 5'- cGGCGCAUGCGCUGUGGuuuuuuuuuccucGGUGUuCuGCc -3' miRNA: 3'- -CCGUGUACGCGACGCU-------------CCACGuGuCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 37304 | 0.78 | 0.264296 |
Target: 5'- gGGcCGCAgGCGCgugGCGAGGUuauccaGCACAGCc -3' miRNA: 3'- -CC-GUGUaCGCGa--CGCUCCA------CGUGUCG- -5' |
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5553 | 3' | -56.4 | NC_001806.1 | + | 138409 | 1.14 | 0.001089 |
Target: 5'- gGGCACAUGCGCUGCGAGGUGCACAGCa -3' miRNA: 3'- -CCGUGUACGCGACGCUCCACGUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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