Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5564 | 5' | -58.7 | NC_001806.1 | + | 131281 | 0.73 | 0.383616 |
Target: 5'- cGCGuGcCCGCGUGGgGGCGCUGUUUg- -3' miRNA: 3'- -UGC-CuGGUGCGCCaCCGCGACAAGuu -5' |
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5564 | 5' | -58.7 | NC_001806.1 | + | 131982 | 0.68 | 0.679805 |
Target: 5'- aGCGccaGACCGgGcCGGUGGCcCUGUUCGc -3' miRNA: 3'- -UGC---CUGGUgC-GCCACCGcGACAAGUu -5' |
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5564 | 5' | -58.7 | NC_001806.1 | + | 132777 | 0.69 | 0.598732 |
Target: 5'- cGCGGugCGggagGCGGUGGCGCgGUggCAu -3' miRNA: 3'- -UGCCugGUg---CGCCACCGCGaCAa-GUu -5' |
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5564 | 5' | -58.7 | NC_001806.1 | + | 139842 | 0.68 | 0.65859 |
Target: 5'- -aGGACCgccugccGCGCGGaGGCGUUGUUa-- -3' miRNA: 3'- ugCCUGG-------UGCGCCaCCGCGACAAguu -5' |
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5564 | 5' | -58.7 | NC_001806.1 | + | 147738 | 0.69 | 0.578557 |
Target: 5'- cACGG-CCGCGCGGgGGCGCgcggGUccCGAc -3' miRNA: 3'- -UGCCuGGUGCGCCaCCGCGa---CAa-GUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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