Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5570 | 5' | -54.1 | NC_001806.1 | + | 69684 | 0.7 | 0.845412 |
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Target: 5'- cUGGCgaGCCUGCGGCg--GGCGcUCGUGg -3' miRNA: 3'- -ACUGg-CGGGUGCUGaaaCUGC-AGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 89595 | 0.7 | 0.843793 |
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Target: 5'- gGACCGCCgGCGGCcgacaggagagUGACGUagccggugggCGUGg -3' miRNA: 3'- aCUGGCGGgUGCUGaa---------ACUGCA----------GCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 50387 | 0.7 | 0.837244 |
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Target: 5'- aGACgGUCCAgUGGCUcucgGugGUCGUGg -3' miRNA: 3'- aCUGgCGGGU-GCUGAaa--CugCAGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 90905 | 0.7 | 0.837244 |
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Target: 5'- cGGCCGCCCACcuGGCgUUUGACGa---- -3' miRNA: 3'- aCUGGCGGGUG--CUG-AAACUGCagcac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 4290 | 0.7 | 0.837244 |
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Target: 5'- gGGCUGCCCggccgugaaGCGGCccgUGGCGUCGc- -3' miRNA: 3'- aCUGGCGGG---------UGCUGaa-ACUGCAGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 131880 | 0.7 | 0.802768 |
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Target: 5'- cGGCCGCCCGCGccGCgacGACGcCGg- -3' miRNA: 3'- aCUGGCGGGUGC--UGaaaCUGCaGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 60493 | 0.7 | 0.802768 |
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Target: 5'- cGGCCGCCgGCGGCaccgaGACGcgcgucUCGUGg -3' miRNA: 3'- aCUGGCGGgUGCUGaaa--CUGC------AGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 92940 | 0.71 | 0.756234 |
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Target: 5'- aGGCCgggGCCCGCGcGCUgaugGACGcCGUGg -3' miRNA: 3'- aCUGG---CGGGUGC-UGAaa--CUGCaGCAC- -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 21094 | 0.73 | 0.686702 |
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Target: 5'- gGAUCGCCCcgcauCGGCgaUGGCGUCGg- -3' miRNA: 3'- aCUGGCGGGu----GCUGaaACUGCAGCac -5' |
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| 5570 | 5' | -54.1 | NC_001806.1 | + | 127125 | 1.09 | 0.004561 |
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Target: 5'- cUGACCGCCCACGACUUUGACGUCGUGg -3' miRNA: 3'- -ACUGGCGGGUGCUGAAACUGCAGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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