Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5584 | 5' | -58.6 | NC_001806.1 | + | 145544 | 0.7 | 0.608659 |
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Target: 5'- cCUGgGGGGGGggaGUgCGGGGGAgGGg -3' miRNA: 3'- aGACgUUCUCCag-CA-GCCCCCUgCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 145671 | 0.68 | 0.688485 |
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Target: 5'- cUCUgGCAcgcGGGGGUgggaaggGUCGGGGGAgGGg -3' miRNA: 3'- -AGA-CGU---UCUCCAg------CAGCCCCCUgCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 147780 | 0.74 | 0.362395 |
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Target: 5'- --cGCGGGGGGccCGgggCGGGGGGCGGa -3' miRNA: 3'- agaCGUUCUCCa-GCa--GCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 148175 | 0.75 | 0.347015 |
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Target: 5'- cUCUGCGugGGGGGgcgCGgggcguccggCGGGGGGCGGg -3' miRNA: 3'- -AGACGU--UCUCCa--GCa---------GCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 148228 | 0.66 | 0.818595 |
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Target: 5'- gCUGCAAGAGacaa-CGGGGGGCGcGa -3' miRNA: 3'- aGACGUUCUCcagcaGCCCCCUGC-Ca -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 151069 | 0.71 | 0.529832 |
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Target: 5'- --cGCGcGGGGGUCG-CGGGGGucGCGGg -3' miRNA: 3'- agaCGU-UCUCCAGCaGCCCCC--UGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 151098 | 0.7 | 0.55906 |
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Target: 5'- --cGCGGG-GGUCG-CGGGGGucGCGGg -3' miRNA: 3'- agaCGUUCuCCAGCaGCCCCC--UGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 151278 | 0.69 | 0.668636 |
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Target: 5'- --cGguGGGGGcCGgggcCGGGGGGCGGc -3' miRNA: 3'- agaCguUCUCCaGCa---GCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 151443 | 0.68 | 0.717901 |
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Target: 5'- --gGCGGGGGG-CGgCGGcGGGGCGGc -3' miRNA: 3'- agaCGUUCUCCaGCaGCC-CCCUGCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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