Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5584 | 5' | -58.6 | NC_001806.1 | + | 56672 | 0.71 | 0.51066 |
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Target: 5'- aUCguuGGGGGGUCGg-GGGGGACGGg -3' miRNA: 3'- -AGacgUUCUCCAGCagCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 93700 | 0.73 | 0.402869 |
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Target: 5'- gCUGUGAGGGGUUG--GGGGGugGGg -3' miRNA: 3'- aGACGUUCUCCAGCagCCCCCugCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 20987 | 0.74 | 0.386337 |
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Target: 5'- gUCgacgGCGGG-GGUCGUCGGGGuccGugGGUc -3' miRNA: 3'- -AGa---CGUUCuCCAGCAGCCCC---CugCCA- -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 12815 | 0.74 | 0.37026 |
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Target: 5'- gUUGUucGGGGUgGcCGGGGGAUGGUa -3' miRNA: 3'- aGACGuuCUCCAgCaGCCCCCUGCCA- -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 147780 | 0.74 | 0.362395 |
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Target: 5'- --cGCGGGGGGccCGgggCGGGGGGCGGa -3' miRNA: 3'- agaCGUUCUCCa-GCa--GCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 148175 | 0.75 | 0.347015 |
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Target: 5'- cUCUGCGugGGGGGgcgCGgggcguccggCGGGGGGCGGg -3' miRNA: 3'- -AGACGU--UCUCCa--GCa---------GCCCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 83902 | 0.75 | 0.339502 |
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Target: 5'- aCUGCAcGGAGGgUCGgCGcGGGGGCGGg -3' miRNA: 3'- aGACGU-UCUCC-AGCaGC-CCCCUGCCa -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 69600 | 0.79 | 0.203837 |
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Target: 5'- cUCUGCGAGGcGGUCGgccugUCGGGGGGCGu- -3' miRNA: 3'- -AGACGUUCU-CCAGC-----AGCCCCCUGCca -5' |
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| 5584 | 5' | -58.6 | NC_001806.1 | + | 121800 | 1.08 | 0.002114 |
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Target: 5'- gUCUGCAAGAGGUCGUCGGGGGACGGUg -3' miRNA: 3'- -AGACGUUCUCCAGCAGCCCCCUGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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