Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5585 | 3' | -55.7 | NC_001806.1 | + | 103784 | 0.66 | 0.908459 |
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Target: 5'- cGGUCUGCUGCaGacCCccGGGCGGCGc -3' miRNA: 3'- -CUAGAUGACGaCccGGuaCCUGUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 57819 | 0.66 | 0.888847 |
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Target: 5'- gGAUCUGCggGCgGGGgUgcGGugGGCGa -3' miRNA: 3'- -CUAGAUGa-CGaCCCgGuaCCugUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 30379 | 0.66 | 0.874602 |
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Target: 5'- aGUCUG-UGCcGGGCgCGUGcGACGGUGg -3' miRNA: 3'- cUAGAUgACGaCCCG-GUAC-CUGUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 52337 | 0.68 | 0.826713 |
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Target: 5'- cGAguauCUGC-GGGCCgcgGUGGACAGCc -3' miRNA: 3'- -CUagauGACGaCCCGG---UACCUGUCGc -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 5071 | 0.68 | 0.809213 |
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Target: 5'- cGUC-GCgGuCUGGGCUcgggGUGGGCGGCGg -3' miRNA: 3'- cUAGaUGaC-GACCCGG----UACCUGUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 74529 | 0.71 | 0.630908 |
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Target: 5'- ---aUGCgGCccUGGGCCAgcagcUGGACAGCGa -3' miRNA: 3'- cuagAUGaCG--ACCCGGU-----ACCUGUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 126114 | 0.72 | 0.568908 |
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Target: 5'- --gCUACUGCgUGGGCCGcgcGGACgacGGCGg -3' miRNA: 3'- cuaGAUGACG-ACCCGGUa--CCUG---UCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 4651 | 0.73 | 0.528389 |
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Target: 5'- cGUCUccGCggcgGCUGGGCCGgcgGGcGCGGCGa -3' miRNA: 3'- cUAGA--UGa---CGACCCGGUa--CC-UGUCGC- -5' |
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| 5585 | 3' | -55.7 | NC_001806.1 | + | 121465 | 1.09 | 0.002508 |
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Target: 5'- aGAUCUACUGCUGGGCCAUGGACAGCGa -3' miRNA: 3'- -CUAGAUGACGACCCGGUACCUGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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