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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5586 | 5' | -52.1 | NC_001806.1 | + | 120069 | 0.66 | 0.985828 |
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Target: 5'- -gGUCGcCUUUAcCGCGCGCAUa--- -3' miRNA: 3'- ugCAGCuGAGAUaGCGCGCGUAacag -5' |
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| 5586 | 5' | -52.1 | NC_001806.1 | + | 131936 | 0.66 | 0.979895 |
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Target: 5'- ---cCGACUCUGUgGCGgGCGcUGUa -3' miRNA: 3'- ugcaGCUGAGAUAgCGCgCGUaACAg -5' |
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| 5586 | 5' | -52.1 | NC_001806.1 | + | 49777 | 0.66 | 0.977556 |
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Target: 5'- cGCGuUCGGg-UUAUCGCGCGUGgggGUCa -3' miRNA: 3'- -UGC-AGCUgaGAUAGCGCGCGUaa-CAG- -5' |
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| 5586 | 5' | -52.1 | NC_001806.1 | + | 36193 | 0.67 | 0.975022 |
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Target: 5'- cGCGUCGauGCUCUGcCGCGCGgGcgGcCa -3' miRNA: 3'- -UGCAGC--UGAGAUaGCGCGCgUaaCaG- -5' |
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| 5586 | 5' | -52.1 | NC_001806.1 | + | 67050 | 0.67 | 0.972286 |
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Target: 5'- cGCaUCGGCggcGUCGCGCGCGUcGUa -3' miRNA: 3'- -UGcAGCUGagaUAGCGCGCGUAaCAg -5' |
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| 5586 | 5' | -52.1 | NC_001806.1 | + | 120966 | 1.11 | 0.005296 |
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Target: 5'- gACGUCGACUCUAUCGCGCGCAUUGUCa -3' miRNA: 3'- -UGCAGCUGAGAUAGCGCGCGUAACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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