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miRNA: 3'- caGGUC-UGCU--AG-AGCCGG-UGCCGGGC- -5'
miRNA: 3'- caGGuCUGCUAGa-GCCGGUGCCGGGc -5'
miRNA: 3'- cAGGUCugCUAG---AGCCGGU--GCCGGGC- -5'
miRNA: 3'- caGGUCUGCUaGaGCCGGU-GCCGGGC- -5'
miRNA: 3'- -CAGGUCUGCUAGAGCCGGUGCCGGGC- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 56638 | 0.74 | 0.329128 |
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Target: 5'- -gCCGGuGCGAccUCaUCGGCCgGCGGCCCGu -3' miRNA: 3'- caGGUC-UGCU--AG-AGCCGG-UGCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 100125 | 0.75 | 0.275239 |
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Target: 5'- -gCCuGGCGGUCcgCGGCUAUGGCCCc -3' miRNA: 3'- caGGuCUGCUAGa-GCCGGUGCCGGGc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 67777 | 0.75 | 0.275239 |
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Target: 5'- cUCCGGugGGUUgugUUGGCCGacUGGCCCGg -3' miRNA: 3'- cAGGUCugCUAG---AGCCGGU--GCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 52568 | 0.76 | 0.228642 |
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Target: 5'- -cCCGGACGA-CaUGGCCAgCGGCCCGg -3' miRNA: 3'- caGGUCUGCUaGaGCCGGU-GCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 118670 | 1.08 | 0.001407 |
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Target: 5'- gGUCCAGACGAUCUCGGCCACGGCCCGg -3' miRNA: 3'- -CAGGUCUGCUAGAGCCGGUGCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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