Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 143413 | 0.66 | 0.728345 |
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Target: 5'- gGUCgCGGcucuuACGAg--CGGCC-CGGCCCGc -3' miRNA: 3'- -CAG-GUC-----UGCUagaGCCGGuGCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 38285 | 0.66 | 0.722619 |
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Target: 5'- -gCCGGGCGAgcggcgucgaguaUCGGCUcCGGUCCGu -3' miRNA: 3'- caGGUCUGCUag-----------AGCCGGuGCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 52903 | 0.66 | 0.719747 |
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Target: 5'- cGUCCGGcGCGcGUCucuggggagccucgaUCGGCCACGGguguggggacccgcCCCGg -3' miRNA: 3'- -CAGGUC-UGC-UAG---------------AGCCGGUGCC--------------GGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 33743 | 0.66 | 0.718788 |
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Target: 5'- -gCCGGcucuGCGGUgUCGGCgGCGGCUgCGg -3' miRNA: 3'- caGGUC----UGCUAgAGCCGgUGCCGG-GC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 75240 | 0.66 | 0.765715 |
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Target: 5'- cGUCCuGGuCGAcg-CGGUgACGGCCCu -3' miRNA: 3'- -CAGGuCU-GCUagaGCCGgUGCCGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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