Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 6088 | 0.66 | 0.755593 |
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Target: 5'- -cCCGGGCGGcggggggcgggucUCUcCGGCgCACauaaaGGCCCGg -3' miRNA: 3'- caGGUCUGCU-------------AGA-GCCG-GUG-----CCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 79520 | 0.66 | 0.756519 |
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Target: 5'- -aCCGGGCG-UCcCGGCCgaaagcacGCGGCCgGc -3' miRNA: 3'- caGGUCUGCuAGaGCCGG--------UGCCGGgC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 136194 | 0.66 | 0.756519 |
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Target: 5'- -aCCAGACGGUCccCGGuCCACaaggggGGCCa- -3' miRNA: 3'- caGGUCUGCUAGa-GCC-GGUG------CCGGgc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 138332 | 0.66 | 0.765715 |
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Target: 5'- -gCCuGACGAUUgaaaagUGGCuCACGGCCa- -3' miRNA: 3'- caGGuCUGCUAGa-----GCCG-GUGCCGGgc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 75240 | 0.66 | 0.765715 |
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Target: 5'- cGUCCuGGuCGAcg-CGGUgACGGCCCu -3' miRNA: 3'- -CAGGuCU-GCUagaGCCGgUGCCGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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