miRNA: 3'- cAGGUCUGcua----GAGCCGG---UGCCGGGc -5'
miRNA: 3'- caGGUCUGcuaGAG----CCGGUgCCGGGC- -5'
miRNA: 3'- caGGUCUGCUagaGCCGGUgCCGGGc -5'
miRNA: 3'- caGGUCUGCUAgagCCGGUgCCGGgC- -5'
miRNA: 3'- -CAGGUcu-GC---UAGAGCCGGUg---CCGGGC- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 147083 | 0.73 | 0.366581 |
|
Target: 5'- uUCCGGGCacacuucCUCGGCCcccGCGGCCCa -3' miRNA: 3'- cAGGUCUGcua----GAGCCGG---UGCCGGGc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 150865 | 0.7 | 0.532854 |
|
Target: 5'- -gCCGGGCcggCUCcgcgGGCCAgGGCCCGg -3' miRNA: 3'- caGGUCUGcuaGAG----CCGGUgCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 151003 | 0.72 | 0.382362 |
|
Target: 5'- -gCCAGGCGGg--CGGCCGaGGCCCa -3' miRNA: 3'- caGGUCUGCUagaGCCGGUgCCGGGc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 151272 | 0.66 | 0.737825 |
|
Target: 5'- -gCCGGGCGGUgggGGCCGgGGCCgGg -3' miRNA: 3'- caGGUCUGCUAgagCCGGUgCCGGgC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 151673 | 0.67 | 0.699473 |
|
Target: 5'- uGUCUAacuCGcuaGUCUCGGCCGggggGGCCCGg -3' miRNA: 3'- -CAGGUcu-GC---UAGAGCCGGUg---CCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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