miRNA: 3'- -CAGGU-CUGCUagaGCCGGUGC--CGGGC- -5'
miRNA: 3'- caGGUCUgCUAGagCCGG----UGCCgGGC- -5'
miRNA: 3'- -CAGGUcuGCUaGAGCCGGUGCCGgGC- -5'
miRNA: 3'- caGGU---CUGCUAGagCCGGUgCCGGGC- -5'
miRNA: 3'- -CAGGUCuGCuagaGCCG--GUGCCGGGC- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 3087 | 0.67 | 0.699473 |
|
Target: 5'- gGUCCGcGGCGGcggCGGCCGCGgaGCUCGg -3' miRNA: 3'- -CAGGU-CUGCUagaGCCGGUGC--CGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 2721 | 0.66 | 0.728345 |
|
Target: 5'- -cCCAGGgGGUCggGGCCcucgGCGGgCCGg -3' miRNA: 3'- caGGUCUgCUAGagCCGG----UGCCgGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 2617 | 0.72 | 0.39859 |
|
Target: 5'- gGUCCG--UGAgCUCGGCCACGGCgCGc -3' miRNA: 3'- -CAGGUcuGCUaGAGCCGGUGCCGgGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 2542 | 0.72 | 0.415251 |
|
Target: 5'- -aCCGcgcGGCGAUCgaGGCCAgGGCCCGc -3' miRNA: 3'- caGGU---CUGCUAGagCCGGUgCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 1730 | 0.69 | 0.581319 |
|
Target: 5'- cGUCCAG-CGc---CGGCagCACGGCCCGg -3' miRNA: 3'- -CAGGUCuGCuagaGCCG--GUGCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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