Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5590 | 5' | -60.2 | NC_001806.1 | + | 119897 | 0.7 | 0.523324 |
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Target: 5'- uGUCCucGGCGc---UGGCCACGGCCCu -3' miRNA: 3'- -CAGGu-CUGCuagaGCCGGUGCCGGGc -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 91894 | 0.7 | 0.523324 |
|
Target: 5'- -aCCAGGCGGUCcccaCGGCCaugggGCGGCUgGa -3' miRNA: 3'- caGGUCUGCUAGa---GCCGG-----UGCCGGgC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 115887 | 0.7 | 0.513861 |
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Target: 5'- cUCCgcGGACGGauucgcUCUCGGUgcguCugGGCCCGa -3' miRNA: 3'- cAGG--UCUGCU------AGAGCCG----GugCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 70039 | 0.7 | 0.501665 |
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Target: 5'- -cCCGGuCGAguucgcccugcgugUgUUGGCCugGGCCCGg -3' miRNA: 3'- caGGUCuGCU--------------AgAGCCGGugCCGGGC- -5' |
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| 5590 | 5' | -60.2 | NC_001806.1 | + | 118670 | 1.08 | 0.001407 |
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Target: 5'- gGUCCAGACGAUCUCGGCCACGGCCCGg -3' miRNA: 3'- -CAGGUCUGCUAGAGCCGGUGCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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