Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5592 | 5' | -53.3 | NC_001806.1 | + | 83825 | 0.67 | 0.953693 |
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Target: 5'- gGUcGGCGCGGGAUCCG---GCACGc- -3' miRNA: 3'- -CAcUUGCGCCCUAGGUgcaUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 99590 | 0.67 | 0.953693 |
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Target: 5'- -gGAACGCGGGcUCCGuCGguagagggGCGCGc- -3' miRNA: 3'- caCUUGCGCCCuAGGU-GCa-------UGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 102140 | 0.69 | 0.865693 |
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Target: 5'- uUGAG-GCGG--UCCGCGUACACGUu -3' miRNA: 3'- cACUUgCGCCcuAGGUGCAUGUGCAu -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 113884 | 0.66 | 0.961297 |
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Target: 5'- gGUuAGCGCGGGGggCCACGaccccgugUACGCGg- -3' miRNA: 3'- -CAcUUGCGCCCUa-GGUGC--------AUGUGCau -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 118000 | 1.07 | 0.00707 |
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Target: 5'- cGUGAACGCGGGAUCCACGUACACGUAc -3' miRNA: 3'- -CACUUGCGCCCUAGGUGCAUGUGCAU- -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 139966 | 0.73 | 0.689978 |
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Target: 5'- -cGAACGCGGGugggacgCCcauAUGUACGCGUAu -3' miRNA: 3'- caCUUGCGCCCua-----GG---UGCAUGUGCAU- -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 147034 | 0.69 | 0.897156 |
|
Target: 5'- cGUGAGgccgccCGCGGGGacggccccggaagucUCCGCGUgGCGCGUc -3' miRNA: 3'- -CACUU------GCGCCCU---------------AGGUGCA-UGUGCAu -5' |
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| 5592 | 5' | -53.3 | NC_001806.1 | + | 147750 | 0.68 | 0.913589 |
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Target: 5'- -gGGGCGCGcGGGUCC-CG-ACGCGg- -3' miRNA: 3'- caCUUGCGC-CCUAGGuGCaUGUGCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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