Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5596 | 3' | -53 | NC_001806.1 | + | 136790 | 0.66 | 0.976234 |
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Target: 5'- --aGCUGC--CGGGuGGUCGCCUCg-- -3' miRNA: 3'- acaUGAUGuuGCCC-UCAGCGGAGagc -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 116811 | 0.66 | 0.974693 |
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Target: 5'- -cUACUGCAgaugcagagugucacGCGGGAGgcCGCCcagCUCa -3' miRNA: 3'- acAUGAUGU---------------UGCCCUCa-GCGGa--GAGc -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 20984 | 0.7 | 0.887686 |
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Target: 5'- --cGCgucgACGGCGGGGGUCGUCgggguccgugggUCUCGc -3' miRNA: 3'- acaUGa---UGUUGCCCUCAGCGG------------AGAGC- -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 130227 | 0.7 | 0.887686 |
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Target: 5'- cGUcGCUGgGGCGGGGGUCGCgcaUCUa- -3' miRNA: 3'- aCA-UGAUgUUGCCCUCAGCGg--AGAgc -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 114091 | 0.71 | 0.83374 |
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Target: 5'- -cUGCUGCAccGCGGGGGuUCGCUUCgaccgCGu -3' miRNA: 3'- acAUGAUGU--UGCCCUC-AGCGGAGa----GC- -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 4034 | 0.75 | 0.59783 |
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Target: 5'- cGUACUGCuGCGcGGcGUCGCCcagCUCGg -3' miRNA: 3'- aCAUGAUGuUGC-CCuCAGCGGa--GAGC- -5' |
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| 5596 | 3' | -53 | NC_001806.1 | + | 116629 | 1.08 | 0.006476 |
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Target: 5'- cUGUACUACAACGGGAGUCGCCUCUCGc -3' miRNA: 3'- -ACAUGAUGUUGCCCUCAGCGGAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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