Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5599 | 3' | -55.1 | NC_001806.1 | + | 91888 | 0.69 | 0.82334 |
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Target: 5'- -aCGUGgaccagGCGGuccCCACGGCCAugGGGCGGc -3' miRNA: 3'- gaGUACa-----UGCU---GGUGCCGGU--CUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 66796 | 0.69 | 0.814702 |
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Target: 5'- -aCAUGccgggAgGAUCACGGCC-GAGCGGg -3' miRNA: 3'- gaGUACa----UgCUGGUGCCGGuCUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 132614 | 0.69 | 0.805897 |
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Target: 5'- uUCAccGUGcCGGCCACGGCC-GAGuCGGc -3' miRNA: 3'- gAGUa-CAU-GCUGGUGCCGGuCUC-GCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 124781 | 0.69 | 0.805897 |
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Target: 5'- ---cUGUACGcCCugGGCCAGAcGCu- -3' miRNA: 3'- gaguACAUGCuGGugCCGGUCU-CGcu -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 128051 | 0.69 | 0.805897 |
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Target: 5'- -cCGUG-GCGGCCACGGCCcccaggugGGGGCc- -3' miRNA: 3'- gaGUACaUGCUGGUGCCGG--------UCUCGcu -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 127937 | 0.7 | 0.740317 |
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Target: 5'- ----aGgcgGCGACCAgGGCgAGGGCGAc -3' miRNA: 3'- gaguaCa--UGCUGGUgCCGgUCUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 94784 | 0.71 | 0.709598 |
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Target: 5'- -cCGUGUugGACCAUGcGCUggcccggacgcggGGGGCGAc -3' miRNA: 3'- gaGUACAugCUGGUGC-CGG-------------UCUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 56841 | 0.72 | 0.670079 |
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Target: 5'- ------cACcGCCGCGGCCAGGGCGGc -3' miRNA: 3'- gaguacaUGcUGGUGCCGGUCUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 109534 | 0.79 | 0.294604 |
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Target: 5'- gCUCGggagccgggggagGUGCG-CCugGGCCAGGGCGAa -3' miRNA: 3'- -GAGUa------------CAUGCuGGugCCGGUCUCGCU- -5' |
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| 5599 | 3' | -55.1 | NC_001806.1 | + | 115290 | 1.09 | 0.003716 |
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Target: 5'- cCUCAUGUACGACCACGGCCAGAGCGAc -3' miRNA: 3'- -GAGUACAUGCUGGUGCCGGUCUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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