Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5607 | 5' | -56.7 | NC_001806.1 | + | 92239 | 0.69 | 0.717873 |
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Target: 5'- --aACCGGUGcUGCgGCGGCgCGUGaUGg -3' miRNA: 3'- cuaUGGCCAU-ACGgCGCCG-GUACcAC- -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 143275 | 0.69 | 0.717873 |
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Target: 5'- gGGUGCgCGGgagGUGUCgGUGGUgGUGGUGg -3' miRNA: 3'- -CUAUG-GCCa--UACGG-CGCCGgUACCAC- -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 49959 | 0.69 | 0.717873 |
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Target: 5'- gGAUGCCGucGUGCgGCuGGCCAUcaacgGGUGg -3' miRNA: 3'- -CUAUGGCcaUACGgCG-CCGGUA-----CCAC- -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 132651 | 0.7 | 0.691806 |
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Target: 5'- uGGUGCgCGGg--GCCGCGGCCGcuuacgccgcgcuccUGGg- -3' miRNA: 3'- -CUAUG-GCCauaCGGCGCCGGU---------------ACCac -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 20628 | 0.7 | 0.690795 |
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Target: 5'- --gGCCGGggguucgaccaacggGCCGCGGCCAcGGg- -3' miRNA: 3'- cuaUGGCCaua------------CGGCGCCGGUaCCac -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 65448 | 0.7 | 0.67762 |
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Target: 5'- --aGCgGGggAUGCCGCGGCCcccgGGUc -3' miRNA: 3'- cuaUGgCCa-UACGGCGCCGGua--CCAc -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 151291 | 0.7 | 0.657233 |
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Target: 5'- --gGCCGGgggGCgGCGGCgGUGGg- -3' miRNA: 3'- cuaUGGCCauaCGgCGCCGgUACCac -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 5595 | 0.71 | 0.575521 |
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Target: 5'- --cGCCGGgggcccgugGCCGCGGCCcguUGGUc -3' miRNA: 3'- cuaUGGCCaua------CGGCGCCGGu--ACCAc -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 30638 | 0.75 | 0.404946 |
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Target: 5'- --gGCCGGgggGgCGCGGCCAgGGUGg -3' miRNA: 3'- cuaUGGCCauaCgGCGCCGGUaCCAC- -5' |
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| 5607 | 5' | -56.7 | NC_001806.1 | + | 111650 | 1.07 | 0.003174 |
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Target: 5'- gGAUACCGGUAUGCCGCGGCCAUGGUGc -3' miRNA: 3'- -CUAUGGCCAUACGGCGCCGGUACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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