Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5616 | 3' | -58.7 | NC_001806.1 | + | 22871 | 0.66 | 0.818209 |
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Target: 5'- uGGcggCcGUCCCGGGGCUGGC-Cg--- -3' miRNA: 3'- -CCaa-GcCAGGGUCCCGACCGuGagac -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 103631 | 0.66 | 0.80966 |
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Target: 5'- gGGUUUGGUCUUGGuGGCcacgGGCACg--- -3' miRNA: 3'- -CCAAGCCAGGGUC-CCGa---CCGUGagac -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 44938 | 0.66 | 0.783116 |
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Target: 5'- ---gUGGUgCCCGGGGCUGGCuagCg- -3' miRNA: 3'- ccaaGCCA-GGGUCCCGACCGugaGac -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 73620 | 0.68 | 0.697361 |
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Target: 5'- --aUCGGUCgCCuGGcGCUGGCGaagCUGa -3' miRNA: 3'- ccaAGCCAG-GGuCC-CGACCGUga-GAC- -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 54493 | 0.68 | 0.657498 |
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Target: 5'- cGGUggGGUCCCugggugGGGGCUGGUgACUg-- -3' miRNA: 3'- -CCAagCCAGGG------UCCCGACCG-UGAgac -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 147699 | 0.69 | 0.617319 |
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Target: 5'- gGGggCGGgcCCCGGaGGC-GGCGCUCg- -3' miRNA: 3'- -CCaaGCCa-GGGUC-CCGaCCGUGAGac -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 56431 | 0.71 | 0.537895 |
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Target: 5'- gGGUccguaaGGUCCCAGGGCggaaGCGC-CUGa -3' miRNA: 3'- -CCAag----CCAGGGUCCCGac--CGUGaGAC- -5' |
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| 5616 | 3' | -58.7 | NC_001806.1 | + | 105585 | 1.09 | 0.001562 |
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Target: 5'- gGGUUCGGUCCCAGGGCUGGCACUCUGu -3' miRNA: 3'- -CCAAGCCAGGGUCCCGACCGUGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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