Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5632 | 3' | -55.9 | NC_001806.1 | + | 3090 | 0.68 | 0.838958 |
Target: 5'- cCGCGgcgGCggCGGCCGCGGagcucgGCAGGc- -3' miRNA: 3'- -GCGUa--UGagGCCGGCGCCa-----UGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 3301 | 0.67 | 0.87738 |
Target: 5'- gGCGUGCggCGGCgGCGGggaaGCGGGg- -3' miRNA: 3'- gCGUAUGagGCCGgCGCCa---UGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 3330 | 0.75 | 0.442075 |
Target: 5'- cCGCGgguccCUCCGGCCGCGGggggcugGCGGGc- -3' miRNA: 3'- -GCGUau---GAGGCCGGCGCCa------UGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 6082 | 0.67 | 0.854938 |
Target: 5'- gGCGgGC-CCgGGCgGCGGgggGCGGGUCu -3' miRNA: 3'- gCGUaUGaGG-CCGgCGCCa--UGUCUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 6146 | 0.66 | 0.92136 |
Target: 5'- cCGCAgacgGCgCCGGCCacgaacgacgggaGCGGcUGCGGAg- -3' miRNA: 3'- -GCGUa---UGaGGCCGG-------------CGCC-AUGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 9858 | 0.68 | 0.812725 |
Target: 5'- cCGCGU--UCCGguacugcGCCGCGGUGCuGAUg -3' miRNA: 3'- -GCGUAugAGGC-------CGGCGCCAUGuCUAg -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 10117 | 0.67 | 0.87738 |
Target: 5'- gCGCAcgGCgcgUCGGCCGgGG-ACAGAc- -3' miRNA: 3'- -GCGUa-UGa--GGCCGGCgCCaUGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 29838 | 0.69 | 0.775654 |
Target: 5'- aCGCAgccccccagCCGGCCGCGGcucggACAGcgCc -3' miRNA: 3'- -GCGUauga-----GGCCGGCGCCa----UGUCuaG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 31091 | 0.66 | 0.910358 |
Target: 5'- gGCG-AC-CCGGCUGCGGgcCGcGGUCc -3' miRNA: 3'- gCGUaUGaGGCCGGCGCCauGU-CUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 40706 | 0.67 | 0.88443 |
Target: 5'- gCGCAgGCUgUGGCCGUGGgcgUACAcccccguaucGAUCa -3' miRNA: 3'- -GCGUaUGAgGCCGGCGCC---AUGU----------CUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 42007 | 0.73 | 0.557293 |
Target: 5'- gGC-UGCUCCgugcuGGCCGCGGggUugGGGUCg -3' miRNA: 3'- gCGuAUGAGG-----CCGGCGCC--AugUCUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 44724 | 0.66 | 0.921915 |
Target: 5'- uCGCGUAUcCCGGCCcCGGUugGaGGg- -3' miRNA: 3'- -GCGUAUGaGGCCGGcGCCAugU-CUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 46418 | 0.67 | 0.854938 |
Target: 5'- gCGCGUcUUCUGcGCCGCGGU-CGGGc- -3' miRNA: 3'- -GCGUAuGAGGC-CGGCGCCAuGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 50892 | 0.71 | 0.689622 |
Target: 5'- gGCGgACUCCgcgcGGCCGCGGaGCacguGGAUCu -3' miRNA: 3'- gCGUaUGAGG----CCGGCGCCaUG----UCUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 66442 | 0.72 | 0.618216 |
Target: 5'- cCGCAgg-UCCGGCUgccaGCGGUACAGccuGUCg -3' miRNA: 3'- -GCGUaugAGGCCGG----CGCCAUGUC---UAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 69648 | 0.69 | 0.758661 |
Target: 5'- gCGCGUAUcaugggUCCGGCCGUGccgACGGAa- -3' miRNA: 3'- -GCGUAUG------AGGCCGGCGCca-UGUCUag -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 70206 | 0.68 | 0.813597 |
Target: 5'- aGCAgcUGCUCCGGgaGCuGUACGuGAUCu -3' miRNA: 3'- gCGU--AUGAGGCCggCGcCAUGU-CUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 73285 | 0.68 | 0.822226 |
Target: 5'- cCGCGUGCUCC-GCCGCccuGGcccGCgAGGUCg -3' miRNA: 3'- -GCGUAUGAGGcCGGCG---CCa--UG-UCUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 84885 | 0.67 | 0.862628 |
Target: 5'- gGCGggACUCUGGgCGUGGUGC--GUCg -3' miRNA: 3'- gCGUa-UGAGGCCgGCGCCAUGucUAG- -5' |
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5632 | 3' | -55.9 | NC_001806.1 | + | 87507 | 0.67 | 0.869372 |
Target: 5'- aCGCGgauCUgCUGGCCGUcguagauGGUGCGGGUg -3' miRNA: 3'- -GCGUau-GA-GGCCGGCG-------CCAUGUCUAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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