Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5632 | 3' | -55.9 | NC_001806.1 | + | 132761 | 0.68 | 0.804803 |
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Target: 5'- cCGgAUGcCUCCuGcCCGCGGUGCGGGa- -3' miRNA: 3'- -GCgUAU-GAGGcC-GGCGCCAUGUCUag -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 29838 | 0.69 | 0.775654 |
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Target: 5'- aCGCAgccccccagCCGGCCGCGGcucggACAGcgCc -3' miRNA: 3'- -GCGUauga-----GGCCGGCGCCa----UGUCuaG- -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 69648 | 0.69 | 0.758661 |
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Target: 5'- gCGCGUAUcaugggUCCGGCCGUGccgACGGAa- -3' miRNA: 3'- -GCGUAUG------AGGCCGGCGCca-UGUCUag -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 50892 | 0.71 | 0.689622 |
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Target: 5'- gGCGgACUCCgcgcGGCCGCGGaGCacguGGAUCu -3' miRNA: 3'- gCGUaUGAGG----CCGGCGCCaUG----UCUAG- -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 66442 | 0.72 | 0.618216 |
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Target: 5'- cCGCAgg-UCCGGCUgccaGCGGUACAGccuGUCg -3' miRNA: 3'- -GCGUaugAGGCCGG----CGCCAUGUC---UAG- -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 88140 | 0.73 | 0.557293 |
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Target: 5'- gGaCGUGCUCCagGGCgGUGGUggACAGGUCg -3' miRNA: 3'- gC-GUAUGAGG--CCGgCGCCA--UGUCUAG- -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 42007 | 0.73 | 0.557293 |
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Target: 5'- gGC-UGCUCCgugcuGGCCGCGGggUugGGGUCg -3' miRNA: 3'- gCGuAUGAGG-----CCGGCGCC--AugUCUAG- -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 3330 | 0.75 | 0.442075 |
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Target: 5'- cCGCGgguccCUCCGGCCGCGGggggcugGCGGGc- -3' miRNA: 3'- -GCGUau---GAGGCCGGCGCCa------UGUCUag -5' |
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| 5632 | 3' | -55.9 | NC_001806.1 | + | 93971 | 1.1 | 0.00236 |
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Target: 5'- cCGCAUACUCCGGCCGCGGUACAGAUCg -3' miRNA: 3'- -GCGUAUGAGGCCGGCGCCAUGUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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