Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5662 | 5' | -54.9 | NC_001806.1 | + | 14124 | 0.66 | 0.926885 |
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Target: 5'- cCCCCUgCCgGACauggUUUGgGGGGCg--- -3' miRNA: 3'- -GGGGAgGGaCUG----AAACaCCCCGauuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 149373 | 0.66 | 0.909519 |
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Target: 5'- aCCCCggUCCUUGugUguuugUUGUgGGGGCg--- -3' miRNA: 3'- -GGGG--AGGGACugA-----AACA-CCCCGauuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 106898 | 0.66 | 0.909519 |
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Target: 5'- cCCCCUCUcuuCUGGCgccuaacGGGGCUGc- -3' miRNA: 3'- -GGGGAGG---GACUGaaaca--CCCCGAUuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 146532 | 0.67 | 0.889956 |
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Target: 5'- uCCCCUCCCcgacacggauUGGCUgguguaGUGGGcGCg--- -3' miRNA: 3'- -GGGGAGGG----------ACUGAaa----CACCC-CGauuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 146049 | 0.67 | 0.88296 |
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Target: 5'- aCCCCcCCCgccCUUUGUGGcGGCcAGAg -3' miRNA: 3'- -GGGGaGGGacuGAAACACC-CCGaUUU- -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 79246 | 0.68 | 0.852721 |
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Target: 5'- cCCCCggCCCUgugGACcUUGuUGGGGCg--- -3' miRNA: 3'- -GGGGa-GGGA---CUGaAAC-ACCCCGauuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 45500 | 0.69 | 0.819176 |
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Target: 5'- aCCCgCCCUGugUggggugagggGUGGGGgUGGAg -3' miRNA: 3'- gGGGaGGGACugAaa--------CACCCCgAUUU- -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 55060 | 0.69 | 0.801315 |
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Target: 5'- aCCCUCCaucACcgcGUGGGGCUGGAg -3' miRNA: 3'- gGGGAGGgacUGaaaCACCCCGAUUU- -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 150228 | 0.7 | 0.763762 |
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Target: 5'- gCCCCUCCCgccGACgcaacaGGGGCUu-- -3' miRNA: 3'- -GGGGAGGGa--CUGaaaca-CCCCGAuuu -5' |
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| 5662 | 5' | -54.9 | NC_001806.1 | + | 74830 | 0.95 | 0.026232 |
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Target: 5'- cCCCCUCCCUGACUUUGU-GGGCUAAAg -3' miRNA: 3'- -GGGGAGGGACUGAAACAcCCCGAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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