Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5675 | 5' | -56.6 | NC_001806.1 | + | 15048 | 0.66 | 0.91023 |
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Target: 5'- cCCgauaaUGGUGGGggGUGUcuucGGgCGACUg -3' miRNA: 3'- -GGag---GCCACCCaaCACAa---CCgGCUGA- -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 99559 | 0.67 | 0.863136 |
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Target: 5'- gCCUCCGGuUGGGauccgGUGgcGGCgGGg- -3' miRNA: 3'- -GGAGGCC-ACCCaa---CACaaCCGgCUga -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 103695 | 0.67 | 0.863136 |
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Target: 5'- cCCUCCguggaGGUGGGg---GUUuGGUCGGCg -3' miRNA: 3'- -GGAGG-----CCACCCaacaCAA-CCGGCUGa -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 3338 | 0.67 | 0.839813 |
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Target: 5'- cCCUCCGGccgcGGGggGcUGgcgGGCCGGg- -3' miRNA: 3'- -GGAGGCCa---CCCaaC-ACaa-CCGGCUga -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 29989 | 0.68 | 0.814837 |
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Target: 5'- cCCUCCGGggGGGUUGggGUuggGGuuGGg- -3' miRNA: 3'- -GGAGGCCa-CCCAACa-CAa--CCggCUga -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 20722 | 0.68 | 0.797364 |
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Target: 5'- cCCgCCGGgGGGgcG-GggGGCCGGCg -3' miRNA: 3'- -GGaGGCCaCCCaaCaCaaCCGGCUGa -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 106616 | 0.7 | 0.712529 |
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Target: 5'- cCCUCCGGUGGaaGUgaugGUGguccccgcgGGCCaGACg -3' miRNA: 3'- -GGAGGCCACC--CAa---CACaa-------CCGG-CUGa -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 85272 | 0.71 | 0.63236 |
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Target: 5'- gCCUCCuu--GGUcGUGUUGGCCGACc -3' miRNA: 3'- -GGAGGccacCCAaCACAACCGGCUGa -5' |
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| 5675 | 5' | -56.6 | NC_001806.1 | + | 67775 | 1.1 | 0.002504 |
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Target: 5'- gCCUCCGGUGGGUUGUGUUGGCCGACUg -3' miRNA: 3'- -GGAGGCCACCCAACACAACCGGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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