miRNA: 3'- -UCUCUG---UCCUugCgGCGGCA-CCCAGG- -5'
miRNA: 3'- -UCUCUGUCCUuGCGG--CGgCACCCaGG- -5'
miRNA: 3'- uCUCUG--UCCUUgCGGCGGCac--CCAGG- -5'
miRNA: 3'- -UCUCUGUCCUUG-------------CGGCGGcACCCAGG- -5'
miRNA: 3'- -UCUCUGUCCUUGCGGCGGCACCCAGG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5692 | 5' | -58.4 | NC_001806.1 | + | 11306 | 0.74 | 0.375206 |
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Target: 5'- gGGAGGCguuGGGGugGgUGUCGUgGGGUCCa -3' miRNA: 3'- -UCUCUG---UCCUugCgGCGGCA-CCCAGG- -5' |
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| 5692 | 5' | -58.4 | NC_001806.1 | + | 27849 | 0.75 | 0.336766 |
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Target: 5'- cGGGGACGGGGcCGCCccGCgGUGGG-CCu -3' miRNA: 3'- -UCUCUGUCCUuGCGG--CGgCACCCaGG- -5' |
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| 5692 | 5' | -58.4 | NC_001806.1 | + | 23945 | 0.75 | 0.329431 |
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Target: 5'- cGGGGCcuGGGGGgGCCGCCGgaguGGUCCg -3' miRNA: 3'- uCUCUG--UCCUUgCGGCGGCac--CCAGG- -5' |
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| 5692 | 5' | -58.4 | NC_001806.1 | + | 56688 | 0.76 | 0.293867 |
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Target: 5'- gGGGGACGGGGGCccgggaaccccggGUCGCUcUGGGUCCu -3' miRNA: 3'- -UCUCUGUCCUUG-------------CGGCGGcACCCAGG- -5' |
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| 5692 | 5' | -58.4 | NC_001806.1 | + | 58789 | 1.12 | 0.001197 |
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Target: 5'- gAGAGACAGGAACGCCGCCGUGGGUCCg -3' miRNA: 3'- -UCUCUGUCCUUGCGGCGGCACCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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