Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5707 | 5' | -55.4 | NC_001806.1 | + | 65316 | 0.66 | 0.940527 |
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Target: 5'- gGCCUcgguggggACGUCggCCGUcuggGUACCGGUa -3' miRNA: 3'- -CGGA--------UGCAGa-GGCAcga-CAUGGCCAc -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 98073 | 0.66 | 0.935751 |
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Target: 5'- gGCC---GUCUCCacGUGC-GUGCCGGUc -3' miRNA: 3'- -CGGaugCAGAGG--CACGaCAUGGCCAc -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 99543 | 0.67 | 0.907707 |
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Target: 5'- gGCCUccGCGUuggaugcCUCCG-GUUGggauCCGGUGg -3' miRNA: 3'- -CGGA--UGCA-------GAGGCaCGACau--GGCCAC- -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 102412 | 0.67 | 0.895694 |
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Target: 5'- cGCCaGCG-CUCCGUGUUcGUAgCGGc- -3' miRNA: 3'- -CGGaUGCaGAGGCACGA-CAUgGCCac -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 75723 | 0.71 | 0.716977 |
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Target: 5'- cGCCgACGUggaggCCGUGCUGgACCGcGUGg -3' miRNA: 3'- -CGGaUGCAga---GGCACGACaUGGC-CAC- -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 4640 | 0.73 | 0.595363 |
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Target: 5'- gGCCgugacgACGUCUCCGcggcgGCUGgGCCGGc- -3' miRNA: 3'- -CGGa-----UGCAGAGGCa----CGACaUGGCCac -5' |
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| 5707 | 5' | -55.4 | NC_001806.1 | + | 51932 | 1.11 | 0.002663 |
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Target: 5'- gGCCUACGUCUCCGUGCUGUACCGGUGg -3' miRNA: 3'- -CGGAUGCAGAGGCACGACAUGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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