miRNA: 3'- aGCCAGCUUAGGCu--ACG-ACAgAGCCu -5'
miRNA: 3'- aGCCAGCUuaGGCuaCGacaGAGCCU- -5'
miRNA: 3'- -AGCCAgCUUAG------GCuaCGACAGAGCCU- -5'
miRNA: 3'- aGCCaGCuuAGGCUACgaCAGaGCCU- -5'
miRNA: 3'- -AGCCAGCUUAGGCUACGACAGAGCCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5744 | 3' | -53.4 | NC_001806.1 | + | 70040 | 0.66 | 0.966923 |
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Target: 5'- cCGGUCGAGUUCGcccUGCgUGUgUUGGc -3' miRNA: 3'- aGCCAGCUUAGGCu--ACG-ACAgAGCCu -5' |
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| 5744 | 3' | -53.4 | NC_001806.1 | + | 37700 | 0.67 | 0.94394 |
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Target: 5'- gCGGUCGA--CCGccGCgcgCUCGGAg -3' miRNA: 3'- aGCCAGCUuaGGCuaCGacaGAGCCU- -5' |
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| 5744 | 3' | -53.4 | NC_001806.1 | + | 126598 | 0.69 | 0.906341 |
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Target: 5'- cUUGGUgGAGUUaaauagCGgcGUUGUCUCGGAg -3' miRNA: 3'- -AGCCAgCUUAG------GCuaCGACAGAGCCU- -5' |
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| 5744 | 3' | -53.4 | NC_001806.1 | + | 113164 | 0.72 | 0.760683 |
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Target: 5'- aCGGgCGggUCCGAUGg-GUCgCGGAa -3' miRNA: 3'- aGCCaGCuuAGGCUACgaCAGaGCCU- -5' |
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| 5744 | 3' | -53.4 | NC_001806.1 | + | 19411 | 1.09 | 0.005397 |
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Target: 5'- cUCGGUCGAAUCCGAUGCUGUCUCGGAu -3' miRNA: 3'- -AGCCAGCUUAGGCUACGACAGAGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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